CRYSTALLIZATION OF EUKARYOTIC E3, LIPOAMIDE DEHYDROGENASE, FROM YEAST, FOR EXHIBITING X-RAY-DIFFRACTION BEYOND 2.5 ANGSTROM RESOLUTION, ANDPRELIMINARY STRUCTURE-ANALYSIS

Lipoamide dehydrogenase, which is a common component of alpha-keto aci d dehydrogenase complexes, has been highly purified from yeast (Saccha romyces cerevisiae) to reveal its structure at higher resolution, New crystals obtained by a desalting method exhibited diffraction beyond 2 .5 Angstrom resolution, The cell dimensions are a = 97.1, b = 158.7, a nd c = 67.9 Angstrom, and the space group is P2(1)2(1)2(1). There is a dimeric enzyme in the asymmetric unit, The crystal structure was solv ed by means of the molecular-replacement technique and refined in a pr eliminary manner.