C. Scheick et al., HUMAN NEUTROPHIL CHEMOTAXIS IN RESPONSE TO DIEPOXIDES OF LINOLENIC ACID, Zeitschrift fur Naturforschung. C, A journal of biosciences, 51(11-12), 1996, pp. 877-882
Diepoxides of linolenic acid were found to be chemoattractants. The co
ncentration that produces 50% of maximal chemotaxis was 4.5 10(-7) mol
/l for human neutrophils when investigated in a chemotaxis test based
on the method of spectrophotometric determination of myeloperoxidase a
ctivity with buffers containing bovine serum albumin. Leukotriene B-4
was used as positive control. The concentration of leukotriene B-4 tha
t produces 50% of maximal chemotaxis was 1.8 nmol/l. No chemotactic ac
tivity was observed when monoepoxides of linoleic or linolenic acid, d
iepoxides of linoleic acid or triepoxides of linolenic acid were used.
Mono-, di- and triepoxides of polyunsaturated fatty acids were synthe
sized with metachloroperbenzoic acid, separated by TLC and HPLC and id
entified by GC/MS.