ANTISALIVARY ANTIBODIES IN PRIMARY SJOGRENS-SYNDROME

Citation
Jc. Atkinson et al., ANTISALIVARY ANTIBODIES IN PRIMARY SJOGRENS-SYNDROME, Journal of oral pathology & medicine, 24(5), 1995, pp. 206-212
Citations number
25
Categorie Soggetti
Dentistry,Oral Surgery & Medicine",Pathology
ISSN journal
09042512
Volume
24
Issue
5
Year of publication
1995
Pages
206 - 212
Database
ISI
SICI code
0904-2512(1995)24:5<206:AAIPS>2.0.ZU;2-K
Abstract
Earlier studies have described an antibody that recognized salivary du ctal epithelium in sera from 15-50% of patients with primary Sjogren's syndrome; however, the specific salivary antigen in those studies was not identified The present study further investigated this unknown sa livary antigen. Twenty-nine of 31 patients (94%) with primary Sjogren' s syndrome demonstrated IgG antinuclear antibodies that bound to an ep ithelial cell line with ductal characteristics derived from a human sa livary gland. Seventy-seven percent of these patients had serum antibo dies that bound to ductal cells of normal human parotid tissue after f ormalin fixation. Western blots of cell extracts, immunofluorescence, and adsorption studies indicated that SS-A/Ro and SS-B/La were the ant igens recognized in the salivary cell line. The pattern of fluorescenc e seen when anti-SS-B/La bound to normal parotid tissue was identical to the fluorescence pattern of the anti-salivary ductal antibodies des cribed in earlier literature.