The objective of this research was to develop on-line measurement and
feed-back control strategies for evaluation and optimization of a gluc
ose-fed CSTR digester. Using methane yield as a direct on-line measure
ment, a model and algorithm were developed and applied for feed-back c
ontrol and adaptive optimization of the digester for temperature. The
model and algorithm were modified and integrated into an expert system
to use methane production rate and dilution rate to automatically det
ect and recover digester abnormal performance resulting from underload
ing, overloading, and addition of an inhibitor (phenol). Online fluoro
metric measurement of NADH, which is a measurement of overall microbia
l activity, was evaluated for its potential contribution to this contr
ol strategy.