COMPARISON OF ENZYMATIC AND CHEMICAL METHODS FOR DETECTING AND DETERMINING TRACE QUANTITIES OF ORGANIC-ACIDS AND AMINO-ACIDS ON CHROMATOGRAMS

Either activation or inhibition of dehydrogenase type enzymes such as isocitrate dehydrogenase (ICDH), lactate dehydrogenase (LDH), glutamat e dehydrogenase (GLDH), alcohol dehydrogenase (AIDH) and aldehyde dehy drogenase (AdDH) has been used to develop new methods for detecting an d determining trace quantities of selected organic acids and aminoacid s on chromatograms. It has been found that the developed methods are s uitable for the determinations of these compounds present in quantitie s from 0.01 to 10 mu g. The sensitivity was increased by 10-100 times compared to that of spectral methods. In addition, the proposed enzyma tic methods are characterized by a good reproducibility, a simple dete rmination procedure within a short period of time, and readily availab le equipment. They are particularly-useful in serial determinations.