DETECTION OF TRACE QUANTITIES OF AMINES WITH THE USE OF ENZYMATIC-REACTIONS AND CHROMATOGRAPHIC TECHNIQUES

A modification has been developed for the detection of trace quantitie s of amines on paper or thin layer chromatograms. The modification of the procedures was based on the inhibition of oxydoreductases by amine compounds. It has been found that the presence of amines in the enzym atic reaction medium clearly affects the activity of enzymes. The chan ge in the activity of the enzyme was proportional to the amount of ana lysed amine. The proposed modification is suitable to detect about 1 n g of amine compound.