A FAST, ECONOMICAL AND EFFICIENT METHOD FOR DNA PURIFICATION BY USE OF A HOMEMADE BEAD COLUMN

A fast, efficient and economical method for purification of DNA from v arious sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the metho d devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable fo r multiple sample handling in the preparation of DNA for sequencing an d other purposes. Protocols are devised for plasmid minipreparation, g el elution and purification of DNA from solutions e.g. enzyme reaction s.