N-GLYCOSYLATED POLYPEPTIDES SYNTHESIZED IN THE EARLY PHASE OF ACNPV INFECTED INSECT CELLS

Infected cell specific polypeptides (ICSP) in Autographa californica n uclear polyhedrosis virus (AcNPV) infected Spodoptera frugiperda cell line, Sf9, were radiolabelled with [C-14] mannose and the labelled pro teins were monitored at various time intervals. A major glycoprotein o f molecular size of 64 kDa appears appears as early as 3h post infecti on (p.i.) which peaks at 12h p.i., but in presence of tunicamycin this protein is labelled. Two other glycoproteins of molecular size 67 kDa and kDa appear at 12h post infection. The glycosylation of these and eight other cellular proteins is also inhibited by tunicamycin. AcNPV propagated in tunicamycin treated Sf9 cells was found to cause delayed infectivity.