NUCLEAR EXPORT OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NUCLEOCYTOPLASMIC SHUTTLE PROTEIN REV IS MEDIATED BY ITS ACTIVATION DOMAIN AND ISBLOCKED BY TRANSDOMINANT NEGATIVE MUTANTS

The human immunodeficiency virus type 1 nucleocytoplasmic shuttle prot ein Rev moves repeatedly between the cytoplasm, a perinuclear zone, th e nucleoli, and nucleoplasmic speckles. In this study, we demonstrated by both indirect immunofluorescence and Western immunoblot analysis t hat nuclear exit of Rev transdominant negative mutants was defective c ompared with that of wild-type Rev, The basic and activation domains o f Rev signal import and export, respectively, of Rev across the nuclea r membrane, In cotransfection experiments, mutants containing mutation s of Rev inhibited the nuclear egress of wild-type Rev, thus revealing a novel transdominant negative phenotype.