L. Briant et al., ANALYSIS OF ENVELOPE SEQUENCE VARIANTS SUGGESTS MULTIPLE MECHANISMS OF MOTHER-TO-CHILD TRANSMISSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1, Journal of virology, 69(6), 1995, pp. 3778-3788
In order to elucidate the molecular mechanisms involved in human immun
odeficiency virus type 1 (HIV-1) mother-to child transmission, we have
analyzed the genetic variation within the V3 hypervariable domain and
flanking regions of the HIV-1 envelope gene in four mother-child tran
smission pairs, Phylogenetic analysis and amino acid sequence comparis
on were performed on cell-associated viral sequences derived from mate
rnal samples collected at different time points during pregnancy, afte
r delivery, and from child samples collected from the time of birth un
til the child was approximately 1 year of age. Heterogeneous sequence
populations were observed to be present in all maternal samples collec
ted during pregnancy and postdelivery. In three newborns, viral sequen
ce populations obtained within 2 weeks after birth revealed a high lev
el of V3 sequence variability, In contrast, V3 sequences obtained from
the fourth child (diagnosed at the age of 1 month) displayed a more r
estricted heterogeneity. The phylogenetic analysis performed for each
mother-child sequence set suggested that several mechanisms may potent
ially be involved in HIV-1 vertical transmission, For one pair, child
sequences were homogeneous and clustered in a single branch within the
phylogenetic tree, consistent with selective transmission of a single
maternal variant. For the other three pairs, the child sequences were
more heterogeneous and clustered in several separate branches within
the tree, In these cases, it appeared likely that more than one matern
al variant was responsible for infection of the child, In conclusion,
no single mechanism can account for mother-to-child HIV-1 transmission
; both the selective transmission of a single maternal variant and mul
tiple transmission events may occur.