ASEPTIC CULTURE OF GYNOPHORES TO OBTAIN PEANUT INTERSECTIONAL HYBRIDS

Cross-incompatibility between cultivated peanuts and their wild relati ves outside the section Arachis has impeded the utilization of many sp ecies possessing high resistances or good qualities. Despite the great efforts made to culture immature ovules or embryos, few hybrid offspr ing have been obtained. In this study, gynophores from Arachis hypogae a L. pollinated with A. glabrata Benth. were cultured and F-1 hybrids seeds were harvested, and F-2 and F-3 generations produced. The charac ters of F-2 generation exhibited a wide range of segregation. Leaf per oxidase isozyme PAGE analysis revealed that the hybrids were quite dif ferent from their parents in relation to band number, width and isozym e activity. The zymograms of the hybrids and their parents were partia lly alike. This verified the authenticity of the hybrids.