LOCALIZATION OF 12-LIPOXYGENASE MESSENGER-RNA IN CULTURED OLIGODENDROCYTES AND ASTROCYTES BY IN-SITU REVERSE-TRANSCRIPTASE AND POLYMERASE CHAIN-REACTION

Authors
BENDANI MK PALLUY O COOKMOREAU J BENEYTOUT JL RIGAUD M VALLAT JM
Citation
Mk. Bendani et al., LOCALIZATION OF 12-LIPOXYGENASE MESSENGER-RNA IN CULTURED OLIGODENDROCYTES AND ASTROCYTES BY IN-SITU REVERSE-TRANSCRIPTASE AND POLYMERASE CHAIN-REACTION, Neuroscience letters, 189(3), 1995, pp. 159-162
Citations number
11
Categorie Soggetti
Neurosciences
Journal title
Neuroscience letters → ACNP
ISSN journal
03043940
Volume
189
Issue
3
Year of publication
1995
Pages
159 - 162
Database
ISI
SICI code
0304-3940(1995)189:3<159:LO1MIC>2.0.ZU;2-2
Abstract
We studied the distribution of 12-lipoxygenase mRNA in glial cells. Fi rst, mRNA was detected from cellular extracts by soluble-phase reverse transcriptase-polymerase chain reaction (RT-PCR). Taking into account that cell culture populations could not be 100% homogenous, we then d eveloped, for the first time, an in situ RT-PCR combined with immunocy tochemistry with cell specific markers. Using this procedure we showed that 12-lipoxygenase mRNA was expressed both in mature oligodendrocyt es and astrocytes.