THE GENE CODING FOR THE MUSTARD TRYPSIN INHIBITOR-2 IS DISCONTINUOUS AND WOUND-INDUCIBLE

The gene coding for the mustard trypsin inhibitor-2 has been isolated from a genomic library and characterized, Comparison of genomic and cD NA sequences indicates that the gene is interrupted by an intron of 19 3 bp, The eukaryotic peculiar regulatory sequences have been detected in the 5' flanking region of the gene, In addition, a decanucleotide h as been detected that is highly similar to the proposed G-box and to t he ABRE motifs required for the gene expression induced by methyl jasm onate and abscissic acid, Northern blot analysis demonstrates that the gene is expressed in immature seeds as well as in wounded leaves.