SEQUENCE-SPECIFIC DNA-BINDING ACTIVITIES OF NUCLEAR MATRIX PROTEINS OF MAMMALIAN LENS EPITHELIAL-CELLS

This study examines matrix and nonmatrix nuclear proteins of the rabbi t lens epithelial cells. The nuclear matrix proteins were isolated by modified Penman technique, which requires presence of detergents and n ucleases, whereas nonmatrix nuclear proteins were obtained by high sal t extraction. The data from these experiments revealed presence of DNA binding activities for SP-1 and OCT-1 proteins in both matrix and non -matrix compartments of rabbit lens epithelial cells. Comparison of th e relative abundance of SP-1 and OCT-1 binding activities in nuclear m atrix and nonmatrix fractions suggest the distribution between these t wo compartments is cell type specific and possibly related to the cont rol of cell growth. (C) 1995 Wiley-Liss, Inc.