M. Shiratsuchi et al., SIMULTANEOUS AND HIGH FERMENTATIVE PRODUCTION OF L-LYSINE AND L-GLUTAMIC ACID USING A STRAIN OF BREVIBACTERIUM-LACTOFERMENTUM, Bioscience, biotechnology, and biochemistry, 59(1), 1995, pp. 83-86
A novel fermentation process for simultaneous and high production of L
-lysine and L-glutamic acid was established. This process was characte
rized by cultivating an L-lysine-producing strain with the cultivating
methods for the production of L-glutamic acid, that is, cultivations
with the addition of surface-active agents or penicillin. By these met
hods, without the addition of ammonium sulfate to culture medium, simu
ltaneous and high production of L-lysine and L-glutamic acid with 1.3-
1.4 fold higher productivity than that of the control was achieved. Va
rious expected merits of this process from the viewpoint of industrial
production of amino acids are discussed. It was suggested from the an
alysis of this newly-found fermentation process that the accumulation
of L-glutamic acid could not be explained only the usual ''leakage mod
el'' through the injury of cell membrane, but it would be necessary to
consider the alterations in L-glutamic acid biosynthetic system.