Ja. Epstein et al., PAX3 INHIBITS MYOGENIC DIFFERENTIATION OF CULTURED MYOBLAST CELLS, The Journal of biological chemistry, 270(20), 1995, pp. 11719-11722
Pax3 is an evolutionarily conserved transcription factor expressed in
the lateral dermomyotome, a region that gives rise to limb muscle prog
enitors. Mutations in Pax-3 account for the mouse mutant Splotch which
develops without limb musculature. We demonstrate that Pax3 can inhib
it myogenic differentiation of C2C12 myoblasts normally induced by exp
osure to low serum. Specific missense mutations that affect the DNA bi
nding characteristics of the two distinct DNA binding domains of Pax3
abolish this effect. Furthermore, we show that Pax3 can inhibit myogen
ic differentiation of 10T1/2 fibroblasts transfected with MyoD, but no
t of 10T1/2 cells transfected with myogenin. This anti-myogenic proper
ty is shared by a PAX3-forkhead fusion protein resulting from a t(2;13
) chromosomal translocation found in pediatric alveolar rhabdomyosarco
mas. These results suggest that Pax3 may suppress the terminal differe
ntiation of migrating limb myoblasts and that the PAX3-forkhead fusion
may contribute to the phenotype of alveolar rhabdomyosarcoma by preve
nting terminal differentiation.