CHARACTERIZATION OF AN ACIDIC POLYSACCHARIDE ISOLATED FROM THE LEAVESOF CORCHORUS-OLITORIUS (MOROHEIYA)

Citation
K. Ohtani et al., CHARACTERIZATION OF AN ACIDIC POLYSACCHARIDE ISOLATED FROM THE LEAVESOF CORCHORUS-OLITORIUS (MOROHEIYA), Bioscience, biotechnology, and biochemistry, 59(3), 1995, pp. 378-381
Citations number
20
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
ISSN journal
09168451
Volume
59
Issue
3
Year of publication
1995
Pages
378 - 381
Database
ISI
SICI code
0916-8451(1995)59:3<378:COAAPI>2.0.ZU;2-V
Abstract
An acidic polysaccharide was isolated from the water-soluble mucilage extracted from dried leaves of Corchorus olitorius, known as Moroheiya in Japan (3.0 g per 100 g). This polysaccharide showed a single peak in a Sepharose CL-6B column, and the specific rotation in H2O at 25 de grees C was +250 degrees. The polysaccharide was rich in uronic acid ( 65%), and consisted of rhamnose, glucose, galacturonic acid, and glucu ronic acid in a molar ratio of 1.0:0.2:0.2:0.9:1.7, in addition to 3.7 % of the acetyl group. A methylation analysis, Smith degradation study and fragmentation analysis suggested that this polysaccharide mainly consisted of O-4 substituted galacturonic acid and glucuronic acid, an d O-2 substituted rhamnose residues, and that most of the (1-->4)-link ed uronic acid residues were substituted at the O-3 position with gluc uronic acid residues. This polysaccharide showed proliferative activit y toward the murine splenocyte.