N-LINKED SUGAR CHAINS OF 350-KDA ROYAL JELLY GLYCOPROTEIN

The structures of N-linked sugar chains from 350 kDa royal jelly glyco protein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chai ns released by hydrazinolysis were done by two-dimensional sugar mappi ng of the corresponding pyridylamino derivatives and 500 MHz H-1-NMR; All four structures were found to fall into the category of oligomanno se-type sugar chains as follows; Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc , Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (M an alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNA c, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 ( Man alpha 1-->2 Man alpha 1-->2 Man alpha 1--3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, and Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1--> 2 Man alpha 1-->3) Man alpha 1-->6(Man alpha 1-->2 Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc.