STUDY ON TOTAL LUMINESCENCE SPECTRA - APPLICATION OF THE MONTE-CARLO METHOD TO 3-DIMENSIONAL SYNCHRONOUS FLUORESCENCE SPECTROMETRY

Three-dimensional synchronous fluorescence spectrometry (TDSFS, a comb ination of synchronous fluorescence spectrometry and three-dimensional fluorescence spectrometry) is a new method which has been developed r ecently. The method has usually been used as an efficient tool to sele ct the best Delta lambda value for synchronous fluorescence spectra. T his paper studies the sensitivity of the method, which was not been do ne in the past. The total fluorescence intensity has been used instead of the conventional single point intensity, calculated by the Monte-C arlo method, as the experimental parameter to determine fluorescein an d tryptophan. The sensitivity of the total fluorescence method is near ly one hundred times better than that of the single point method. The new method has been used to simultaneously determine naphthalene, pyre ne and perylene successfully. The mechanism of the method has also bee n studied.