Fg. Sanchez et al., VARIABLE-ANGLE SCANNING FLUORESCENCE SPECTROMETRY FOR THE SIMULTANEOUS DETERMINATION OF 3 DIURETIC DRUGS, Analytica chimica acta, 306(2-3), 1995, pp. 313-321
The applicability of variable-angle synchronous scanning (VASS) fluore
scence spectroscopy has been demonstrated for the resolution of mixtur
es of three diuretics (furosemide, triamterene, piretamide) with close
ly overlapping fluorescence profiles. This technique permits linear or
non-linear paths to be scanned at preselected angles through the exci
tation-emission matrix by scanning both monochromators at different sp
eeds, in order to obtain the best variable-angle scanning spectra (hig
hest signal values and interference-free bands). Such an approach has
permitted the simultaneous determination of the three compounds at the
mu g to ng ml(-1) level, with a relative standard deviation less than
or equal to 5% (n = 10). When applied to commercial pharmaceutical do
sage forms, recoveries of the original drug between 98-101% have been
obtained.