VARIABLE-ANGLE SCANNING FLUORESCENCE SPECTROMETRY FOR THE SIMULTANEOUS DETERMINATION OF 3 DIURETIC DRUGS

Citation
Fg. Sanchez et al., VARIABLE-ANGLE SCANNING FLUORESCENCE SPECTROMETRY FOR THE SIMULTANEOUS DETERMINATION OF 3 DIURETIC DRUGS, Analytica chimica acta, 306(2-3), 1995, pp. 313-321
Citations number
32
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032670
Volume
306
Issue
2-3
Year of publication
1995
Pages
313 - 321
Database
ISI
SICI code
0003-2670(1995)306:2-3<313:VSFSFT>2.0.ZU;2-R
Abstract
The applicability of variable-angle synchronous scanning (VASS) fluore scence spectroscopy has been demonstrated for the resolution of mixtur es of three diuretics (furosemide, triamterene, piretamide) with close ly overlapping fluorescence profiles. This technique permits linear or non-linear paths to be scanned at preselected angles through the exci tation-emission matrix by scanning both monochromators at different sp eeds, in order to obtain the best variable-angle scanning spectra (hig hest signal values and interference-free bands). Such an approach has permitted the simultaneous determination of the three compounds at the mu g to ng ml(-1) level, with a relative standard deviation less than or equal to 5% (n = 10). When applied to commercial pharmaceutical do sage forms, recoveries of the original drug between 98-101% have been obtained.