GENE ORGANIZATION AND LOW REGIOSPECIFICITY IN AROMATIC-RING HYDROXYLATION OF A BENZENE MONOOXYGENASE OF PSEUDOMONAS-AERUGINOSA JI104

Citation
A. Kitayama et al., GENE ORGANIZATION AND LOW REGIOSPECIFICITY IN AROMATIC-RING HYDROXYLATION OF A BENZENE MONOOXYGENASE OF PSEUDOMONAS-AERUGINOSA JI104, Journal of fermentation and bioengineering, 82(5), 1996, pp. 421-425
Citations number
20
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
ISSN journal
0922338X
Volume
82
Issue
5
Year of publication
1996
Pages
421 - 425
Database
ISI
SICI code
0922-338X(1996)82:5<421:GOALRI>2.0.ZU;2-T
Abstract
A novel benzene monooxygenase gene cluster has been cloned from Pseudo monas aeruginosa J104 which was isolated from soil as a benzene degrad er. The nucleotide sequence of this gene cluster was found to be highl y homologous to those of other toluene monooxygenase gene clusters. Th is multicomponent monooxygenase also has the capability to catalyze th e hydroxylation of various alkylated aromatic hydrocarbons. The low re giospecific hydroxylation was observed when toluene, o-xylene, ethyl b enzene and n-propyl benzene were used as substrates.