THE GENOMIC ORGANIZATION OF THE GENE ENCODING A NITRATE-INDUCIBLE FERREDOXIN-NADP(+) OXIDOREDUCTASE FROM RICE ROOTS

A genomic clone of the gene encoding a nitrate-inducible ferredoxin-NA DP(+) oxidoreductase (FNR) from rice (Oryza sativa L.) roots has been isolated and its nucleotide sequence determined. The clone contains 38 97 nucleotides of the gene which consists of six exons interrupted by five introns. The transcription start site was determined by primer ex tension analysis which locates 64 bp upstream of the ATG translation i nitiation codon. The 5'-flanking region contains canonical TATA- and C AAT-boxes, and a potential Sp1-binding site. Four ATCAA(A/C) and two i nverted TTTGAT sequences are localized in the promoter region and a TG TAA motif occurs three times in the 3'-untranslated region. No signifi cant similarity was found when the 5' flanking region was compared wit h that of the photosynthetic FNR gene.