MOLECULAR-CLONING AND EXPRESSION OF A CDNA-ENCODING A PROTEIN-TYROSINE-PHOSPHATASE FROM MOUSE-BRAIN

Authors
PARK KS KIM YW KIM CW KANG DO BYUN SM
Citation
Ks. Park et al., MOLECULAR-CLONING AND EXPRESSION OF A CDNA-ENCODING A PROTEIN-TYROSINE-PHOSPHATASE FROM MOUSE-BRAIN, Biochemistry and molecular biology international, 40(5), 1996, pp. 1025-1032
Citations number
20
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
40
Issue
5
Year of publication
1996
Pages
1025 - 1032
Database
ISI
SICI code
1039-9712(1996)40:5<1025:MAEOAC>2.0.ZU;2-2
Abstract
A mouse brain cDNA library was screened with a radioisotope-labeled pr obe of human placental protein tyrosine phosphatase. The isolated clon e (MBPTP1B) was found to contain an open reading frame of 1,296 nucleo tides as well as 5' (709 nucleotides) and 3' (341 nucleotides) non-cod ing regions. This cDNA encodes a PTP of 432 amino acids having a mass of 49,563 daltons and exhibiting 83% and 93.5% sequence identity to th at of human PTP1B and rat PTP1, respectively. The expression of the cD NA in yeast was identified by western blot analysis and PTP activity a ssay.