A NOVEL METHOD OF PREPARING RAT-MONOCLONAL ANTIBODY-PRODUCING HYBRIDOMAS BY USING RAT MEDIAL ILIAC LYMPH-NODE CELLS

A novel method of preparing hybridomas producing rat monoclonal antibo dies was established. The enlarged medial iliac lymph nodes from rats injected via hind footpads with an emulsion of antigen and Freund's ad juvant were used for cell fusion. Ovalbumin was used as a representati ve antigen. The incidence of hybridomas producing antibody of interest with this method was about 10 times higher than that of hybridomas wi th the conventional method using mouse or rat spleen cells. The averag e percentages of hybridomas producing IgG1, IgG2a, IgG2b and IgG2c wer e 37.1%, 47.0%, 15.9% and 0.0%, respectively. A single injection with antigen was sufficient for immunization in this method.