EFFECT OF THE PARASITE ENZYME, HYPODERMIN-A, ON BOVINE LYMPHOCYTE-PROLIFERATION AND INTERLEUKIN-2 PRODUCTION VIA THE PROSTAGLANDIN PATHWAY

Citation
I. Nicolasgaulard et al., EFFECT OF THE PARASITE ENZYME, HYPODERMIN-A, ON BOVINE LYMPHOCYTE-PROLIFERATION AND INTERLEUKIN-2 PRODUCTION VIA THE PROSTAGLANDIN PATHWAY, Immunology, 85(1), 1995, pp. 160-165
Citations number
25
Categorie Soggetti
Immunology
Journal title
ISSN journal
00192805
Volume
85
Issue
1
Year of publication
1995
Pages
160 - 165
Database
ISI
SICI code
0019-2805(1995)85:1<160:EOTPEH>2.0.ZU;2-W
Abstract
The immune function of cattle infected with a primary infestation of H ypoderma lineatum is impaired during the first instar migration of the larvae. Hypodermin A (HA) is an enzyme secreted by the larvae that is implicated in immunosuppression. The response of bovine peripheral bl ood mononuclear cells (PBMC) to HA was examined in this study. HA bloc ked their proliferation in response to phytohaemagglutinin (PHA) and i ts effect was enhanced when cells were preincubated with HA before act ivation. This suggests that HA affects the lymphocyte commitment to bl astogenesis during the early stages of their activation. HA also markl y reduced the production of interleukin-2 (IL-2) in PHA-stimulated bov ine PBMC cultures. Furthermore, indomethacin, which inhibits prostagla ndin (PG) synthesis, blocked the immunosuppressive effect of HA on the PBMC proliferative response. The concentration of PGE(2) in medium of PBMC or PMA-stimulated monocyte cultures was increased by incubation with HA, Thus, the HA appeared to act by reducing IL-2 production via a prostaglandin-dependent pathway.