DETERMINATION OF HEMOGLOBIN ADDUCTS OF ARYLAMINES IN HUMANS

Aromatic amines and nitroarenes are important occupational and environ mental pollutants. It is therefore essential to have fast and reliable methods to monitor human exposure. In rat experiments it has been sho wn that several arylamines form hydrolyzable hemoglobin adducts. Hemog lobin adducts are dosimeters for the internal dose and possibly for DN A modification at the site of tumor formation. In the present paper we introduce a new efficient method to quantify hemoglobin adducts. Prec ipitated hemoglobin was hydrolyzed in base in the presence of the reco very standards d(5)-aniline, d(4)-2-methylaniline, d(4)-3-methylanilin e, d(4)-4-methylaniline, d(3)-2,4-dimethylaniline, C-13(6)-4-chloroani line, d(9)-4-aminobiphenyl, and 4'-fluoro-4-aminobiphenyl. The hydroly sate was extracted with hexane, derivatized with pentafluoropropionic anhydride (PFPA) and analyzed by GC-MS with negative chemical ionizati on. In one run over twenty amines can be determined. The method has be en applied to human studies.