IN-111-OXINE LABELING OF TUMOR-CYTOTOXIC MACROPHAGES GENERATED IN-VITRO FROM CIRCULATING BLOOD MONOCYTES - AN IN-VITRO EVALUATION

The labelling of ex-vivo activated macrophages with doses of In-111-ox ine sufficient for gamma camera imaging does not damage cell viability or the functional competence of cells (secretion of tumour necrosis f actor-alpha and interleukin-6). The mean labelling efficiency was abou t 84%. Release of In-111 equivalent to 15% occurred over 24 h under ce ll culture conditions, indicating good stability of the radioactive ce ll label. In-111-oxine-labelled macrophages are a suitable tool for bi okinetic studies of adoptive immunotherapy.