POLYPEPTIDES FROM MURINE PERITONEAL-MACRO PHAGES THAT BIND GLUCOSAMINYLMURAMYLDIPEPTIDES

Authors
GOLOVINA TN SUMAROKA MV SAMOKHVALOVA LV SHEBZUKHOV YV MAKAROV EA NESMEYANOV VA
Citation
Tn. Golovina et al., POLYPEPTIDES FROM MURINE PERITONEAL-MACRO PHAGES THAT BIND GLUCOSAMINYLMURAMYLDIPEPTIDES, Bioorganiceskaa himia, 21(4), 1995, pp. 268-274
Citations number
19
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
21
Issue
4
Year of publication
1995
Pages
268 - 274
Database
ISI
SICI code
0132-3423(1995)21:4<268:PFMPPT>2.0.ZU;2-V
Abstract
By means of radioligand analysis, murine peritoneal macrophages were s hown to express several hundreds cell surface high-affinity GMDP-bindi ng sites with a binding constant 350 pM. Photoaffinity labeling follow ed by SDS-PAGE enabled us to identify inside these cells 32 - 34 and 3 8 kDa proteins, specifically binding GMDP. Proteins 32 - 34 kDa were a lso detected by Western blotting analysis using biotinylated conjugate of polyacrylamide with immobilized GMDP-Lys [(GMDP-Lys)-PAA-(Bi)] in cell lysate of murine peritoneal macrophages.