FAST-ION BOMBARDMENT MASS-SPECTROMETRY OF SANGUINARINE AND COPTISINE FROM CHELIDONIUM-MAJUS L CALLUS CELLS

This paper reports the identification of sanguinarine and coptisine is olated from the crude extract of callus culture of Chelidonium majus b y positive fast ion bombardment (FIB+) mass spectrometry (MS) and FIB tandem mass spectrometry (MS-MS). Callus was cultivated on Gamborg B5 medium for three weeks at 25-degrees-C and extracted with 70% ethanol . The alkaloids were extracted into chloroform, following ion pair for mation, and then separated on a silica gel column. The presence of san guinarine and coptisine was initially determined from chromatographic analysis, and the structures of the compounds were confirmed by MS. MS -MS analysis was not suitable for coptisine since this compound did no t produce daughter ions.