Cs. Teng, BIOLOGICAL-ACTIVITY IN THE REPOPULATING RAT SPERMATOCYTE AFTER THE WITHDRAWAL OF GOSSYPOL TREATMENT .5. INHIBITION AND RECOVERY OF MICROTUBULAR DYNEIN, Contraception, 51(5), 1995, pp. 313-318
After oral administration with gossypol acetic acid for various rimes,
young male rats developed a low content of microtubular (or cytoplasm
ic) dynein in the spermatogenic cells, e.g., spermatids and primary sp
ermatocytes. The content of dynein in the cells was measured by enzyme
-linked immunosorbent assay (ELISA) using monoclonal anti-dynein antib
ody. The results were expressed as ng dynein/10(6) cells and compared
with those of the control rats. After gossypol treatment for 8, 12, an
d 19 weeks, the content of dynein in spermatids was reduced by 61%, 70
%, and 68%, respectively; whereas, the amount of dynein in primary spe
rmatocytes was reduced by 37%, 44%, and 31%, respectively. The microtu
bular dynein associated with spermatids was more vulnerable to gossypo
l than that of the primary spermatocytes. Immunofluorescent staining t
echnique confirmed the finding that the control cells have more dynein
than that of the drug-treated cells. Eight weeks after the withdrawal
of the drug treatment, the content of dynein in spermatids and primar
y spermatocytes was fully recovered. The possible effects of this chan
ge in conjunction with the function of microtubules during spermatogen
esis and sperm motility are discussed.