PEROXIDATIVE XENOBIOTIC OXIDATION BY PARTIALLY PURIFIED PEROXIDASE AND LIPOXYGENASE FROM HUMAN FETAL TISSUES AT 10 WEEKS OF GESTATION

1. Present study reports the ability of partially purified peroxidase and lipoxygenase from human fetal tissues at 10 weeks of gestation to oxidize selected xenobiotics in vitro. 2. Peroxidase was found to oxid ize four different chemicals in the presence of H2O2. Sodium azide and potassium cyanide inhibited peroxidase activity towards guaiacol in a concentration-dependent manner. 3. The dioxygenase and co-oxidase act ivities of lipoxygenase towards linoleic acid and four model xenobioti cs, respectively, were observed. Both the catalytic activities of lipo xygenase were significantly inhibited by <1.0 mu M nordihydroguaiareti c acid. 4. These findings suggest that peroxidase and lipoxygenase may be important pathways for peroxidative xenobiotic oxidation in human fetal tissues.