SUBCUTANEOUS TISSUE REACTION TO SYNTHETIC AUDITORY OSSICLE (APACERAM(R)) IN RATS

A study was carried out in order to obtain further information about t he soft tissue response to thin Apaceram(R) discs of dense hydroxyapat ite (HA) implanted in rats for various periods of time between one day and 10 months. The Apaceram(R) discs were implanted subcutaneously in to the interscapular region of 33 rats. A sham operation was performed on eight rats used as controls. Decalcified histological sections sta ined with haematoxylin and eosin and Mallory's azan were examined and the different cell types found around the implants were counted. It wa s found that an acute inflammatory reaction occurred after one day and disappeared at about two weeks after implantation. In the test groups , macrophages and lymphocytes disappeared about one week later, and no inflammatory reaction was observed from one to three months. However, a tissue reaction occurred at six months with the appearance of macro phages and lymphocytes, and decreased gradually at 10 months. Meanwhil e, a few foreign body giant cells at the Apaceram(R)-tissue interface and a thick layer of fibrous connective tissue around the Apaceram(R) disc were observed at 10 months. No osteogenesis was observed in any s pecimen. The results obtained so far suggest that Apaceram(R) is still a useful material for reconstructive surgery, despite the possible ap pearance of a slight macrophage reaction at six months.