Dk. Garcia et Jah. Benzie, RAPD MARKERS OF POTENTIAL USE IN PENAEID PRAWN (PENAEUS-MONODON) BREEDING PROGRAMS, Aquaculture, 130(2-3), 1995, pp. 137-144
Single, short generic primers of arbitrary sequence used to amplify DN
A from Penaeus monodon gave three polymorphic markers out of the total
of 48 bands that amplified. This level of polymorphism is similar to
that displayed by other taxa (6-7%) and suggests randomly amplified po
lymorphic DNA (RAPD) approaches will be as useful in providing markers
for prawn breeding programs as they have been for other species. Fift
y offspring from each of the six families screened for the three polym
orphic markers demonstrated that all the markers conformed to Mendelia
n expectations for band presence/absence, consistent with a model of c
omplete or co-dominance where dominant homozygotes and heterozygotes d
isplay a band but homozygote recessives do not. A fourth primer amplif
ied a band in almost all of the progeny but in none of the adults, and
was probably derived from algal or bacterial epicommensals contaminat
ing the sample, emphasising the need to establish the inheritance of R
APD polymorphisms prior to their use as genetic markers.