ANALYSIS OF STRUCTURAL DEFECTS OF POLY(G) POLY(C) COMPLEX

The interaction between poly(C) and poly(G).poly(C) was studied by dif ferential impulse polarography. It was shown that binding of poly(C) t o poly(G).poly(C) hinders its interaction with the electrode. The low concentration of poly(C) in the presence of poly(G).poly(C) was attrib uted to its interaction with unpaired G residues of poly(G). To determ ine the concentration of these residues, the fluorescence of Tb3+ was measured in the presence of poly(G).poly(C). It was found that denatur ation of poly(G).poly(C) followed by renaturation reduces the number o f defects in the poly(G) molecule.