E. Kirschning et al., A SULFATIDE-REACTIVE HUMAN MONOCLONAL-ANTIBODY OBTAINED FROM A MULTIPLE-SCLEROSIS PATIENT SELECTIVELY BINDS TO THE SURFACE OF OLIGODENDROCYTES, Journal of neuroimmunology, 56(2), 1995, pp. 191-200
In a previous paper, we described the production of a sulfatide-reacti
ve IgM antibody-secreting B cell line that was obtained by Epstein-Bar
r virus transformation of peripheral B cells from a patient with multi
ple sclerosis (MS) (Uhlig and Dernick, 1989). In the present study, we
demonstrate that this human monoclonal antibody (humAb) DS1F8 selecti
vely binds to the surface of living oligodendrocytes in mixed brain ce
ll cultures of newborn rats. Since a mouse mAb reactive with sulfatide
was shown to inhibit oligodendrocyte progenitor differentiation, auto
antibodies with binding specificities similar to DS1F8 could play a ro
le in the demyelinating process in the CNS.