A SULFATIDE-REACTIVE HUMAN MONOCLONAL-ANTIBODY OBTAINED FROM A MULTIPLE-SCLEROSIS PATIENT SELECTIVELY BINDS TO THE SURFACE OF OLIGODENDROCYTES

Authors
KIRSCHNING E RUTTER G UHLIG H DERNICK R
Citation
E. Kirschning et al., A SULFATIDE-REACTIVE HUMAN MONOCLONAL-ANTIBODY OBTAINED FROM A MULTIPLE-SCLEROSIS PATIENT SELECTIVELY BINDS TO THE SURFACE OF OLIGODENDROCYTES, Journal of neuroimmunology, 56(2), 1995, pp. 191-200
Citations number
50
Categorie Soggetti
Neurosciences,Immunology
Journal title
Journal of neuroimmunology → ACNP
ISSN journal
01655728
Volume
56
Issue
2
Year of publication
1995
Pages
191 - 200
Database
ISI
SICI code
0165-5728(1995)56:2<191:ASHMOF>2.0.ZU;2-P
Abstract
In a previous paper, we described the production of a sulfatide-reacti ve IgM antibody-secreting B cell line that was obtained by Epstein-Bar r virus transformation of peripheral B cells from a patient with multi ple sclerosis (MS) (Uhlig and Dernick, 1989). In the present study, we demonstrate that this human monoclonal antibody (humAb) DS1F8 selecti vely binds to the surface of living oligodendrocytes in mixed brain ce ll cultures of newborn rats. Since a mouse mAb reactive with sulfatide was shown to inhibit oligodendrocyte progenitor differentiation, auto antibodies with binding specificities similar to DS1F8 could play a ro le in the demyelinating process in the CNS.