Ld. Masnatta et al., DETERMINATION OF BEZAFIBRATE, CIPROFIBRATE AND FENOFIBRIC ACID IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 687(2), 1996, pp. 437-442
Citations number
10
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A selective high-performance liquid chromatographic method to assess e
ither bezafibrate, ciprofibrate or fenofibric acid plasma levels is de
scribed. Drugs are extracted with diethyl ether, after acidification w
ith HCl. An isocratic acetonitrile-0.02 M H3PO4 (55:45) mobile phase,
a C-18 (5 mu m) column and UV detection are used. The LOQ found was 0.
25 mu g/ml for the three fibrates. Intra- and inter-assay accuracy ran
ges were 90-107% and 82-111%; 96-115% and 94-107%; 94-114% and 94-126%
for bezafibrate, ciprofibrate and fenofibric acid, respectively. Intr
a- and inter-assay precision (C.V.% ranges) were 1.72-3.06% and 2.66-7
.67%; 1.88-4.64% and 0.62-2.99%; 1.26-4.69% and 3.56-7.17% for the thr
ee fibrates studied. Its sensitivity, accuracy and precision make it a
useful tool for monitoring plasma levels of these drugs in a clinical
setting and for research purposes.