ANALYSIS OF BASIC ANTIMALARIAL-DRUGS BY CZE .2. VALIDATION AND APPLICATION TO BIOANALYSIS

This report describes some of the quantitative aspects of the CZE sepa ration of proguanil, chloroquine and their respective metabolites, the separations of which, by CE and MEKC, were reported in Part 1. Result s obtained on the precision of migration time and peak areas using the alternative injection methods of vacuum and electrokinetic are descri bed and discussed. The increase in concentration sensitivity using ele ctrokinetic injection with an organic injection solvent reported in Pa rt 1 is confirmed and the resultant limits of detection in urine repor ted. An assay method for these compounds in urine is described which i ncorporates a pretreatment stage of solid phase extraction and the mai n analytical parameters used in the validation of such an assay are re ported. The limitation of the sample pretreatment used when applied to matrices of plasma and saliva are reported and discussed in the conte xt of the electrokinetic injection method used.