ANALYSIS OF UNCONJUGATED MORPHINE, CODEINE, NORMORPHINE AND MORPHINE AS GLUCURONIDES IN SMALL VOLUMES OF PLASMA FROM CHILDREN

Authors
WATSON DG SU Q MIDGLEY JM DOYLE E MORTON NS
Citation
Dg. Watson et al., ANALYSIS OF UNCONJUGATED MORPHINE, CODEINE, NORMORPHINE AND MORPHINE AS GLUCURONIDES IN SMALL VOLUMES OF PLASMA FROM CHILDREN, Journal of pharmaceutical and biomedical analysis, 13(1), 1995, pp. 27-32
Citations number
19
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
13
Issue
1
Year of publication
1995
Pages
27 - 32
Database
ISI
SICI code
0731-7085(1995)13:1<27:AOUMCN>2.0.ZU;2-K
Abstract
A sensitive method for the analysis of unconjugated morphine, codeine, normorphine and total morphine after hydrolysis of glucuronide conjug ates is described. The method was applicable to 50-mu l volumes of pla sma. The analytes were converted to heptafluorobutyryl (HFB) derivativ es before analysis by gas chromatography-negative ion chemical ionizat ion mass spectrometry. Morphine and codeine were quantified against th eir [H-2(3)]-isotopomers. Linearity, precision and accuracy were quite acceptable (in the 10(-10)-10(-9) g range), and the absolute limits o f detection were <1 pg.