RAPID METHOD FOR THE ANALYSIS OF ITRACONAZOLE AND HYDROXYITRACONAZOLEIN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
COMPAS D TOUW DJ DEGOEDE PNFC
Citation
D. Compas et al., RAPID METHOD FOR THE ANALYSIS OF ITRACONAZOLE AND HYDROXYITRACONAZOLEIN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 687(2), 1996, pp. 453-456
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
687
Issue
2
Year of publication
1996
Pages
453 - 456
Database
ISI
SICI code
Abstract
An assay for the determination of itraconazole and its active metaboli te hydroxyitraconazole in serum has been developed, using ketoconazole as internal standard. The procedure involved a one-step liquid-liquid extraction of the drug, its metabolite and the internal standard, fol lowed by their separation by reversed-phase HPLC. In this paper the va lidation of this method is described.