IN-SITU HYBRIDIZATION TO METAPHASE CHROMOSOMES IN 6 SPECIES OF PHASEOLUS AND VIGNA USING RIBOSOMAL DNA AS THE PROBE

In situ hybridization with a biotin-labeled rice ribosomal DNA (rDNA) probe to the somatic metaphase chromosomes of six species of Phaseolus and Vigna (P. angularis, P. calcaratus, P. coccineus, P. vulgaris, V. sesquipedalis and V. sinensis) was done to determine the sites of rDN A, Hybridization signals were present in the terminal and subterminal chromosome regions of each of the six species. The number of rDNA site s was two in P. angularis and P. calcaratus, four in P. coccineus and P. vulgaris, and six in V. sesquipedalis and V. sinensis.