The chromosome-damaging effects of urine concentrates (UCs) from tobac
co plus areca nut (T/AN) chewers (a highly popular habit and a major r
isk factor for oral cancer in India) were evaluated on Chinese hamster
ovary (CHO) cells employing two cytogenetic end-points, namely chromo
some aberration (CA) and sister chromatid exchange (SCE) frequencies.
Urine creatinine levels were comparable between controls and T/AN chew
ers. CA and SCE frequencies in CHO cells were found to be elevated sig
nificantly (P < 0.001) following treatment with UCs prepared from T/AN
chewers (UC-T/AN chewers) as well as with UCs of non-chewer controls
(UC-control subjects). Moreover, elevation of these two parameters by
UC-T/AN chewers was significantly higher in comparison to that of UC-c
ontrols. The results of the present study indicated that besides the o
ral cavity, which is a target organ for T/AN chewers, mutagens/carcino
gens in tobacco and areca nut might be playing a causative role in can
cer of the urinary bladder as well.