C. Simonmateo et al., MAPPING AND SEQUENCE OF THE GENE ENCODING PROTEIN P17, A MAJOR AFRICAN SWINE FEVER VIRUS STRUCTURAL PROTEIN, Virology, 206(2), 1995, pp. 1140-1144
The gene encoding protein p17, a major structural protein of African s
wine fever virus, has been mapped and sequenced. Protein p17 was purif
ied from dissociated virus by reverse-phase HPLC and the amino acid se
quence of a peptide obtained after digestion of protein p17 with cyano
gen bromide was determined by automated Edman degradation. To map the
gene encoding protein p17, a mixture of 17-mer oligonucleotides based
upon a part of the amino acid sequence was hybridized to cloned Africa
n swine fever virus DNA restriction fragments. This allowed the locati
on of the gene in the fragment EcoRI D of the viral genome. The nucleo
tide sequence of a SalI/KpnI fragment revealed an open reading frame d
esignated D117R encoding a protein of 117 amino acids with a deduced m
olecular mass of about 13,000 Da. A transcriptional analysis revealed
that the p17 gene is expressed late in the viral infection cycle. (C)
1995 Academic Press, Inc.