We have determined the high resolution crystal structure of the A doma
in from the a chain of integrin CR3. The domain adopts a classic alpha
/beta ''Rossmann'' fold and contains an unusual Mg2+ coordination site
at its surface. One of the coordinating ligands is the glutamate side
chain from another A domain molecule. We suggest that this site repre
sents a general metal ion-dependent adhesion site (MIDAS) for binding
protein ligands. We further propose that the beta subunits of integrin
s contain a MIDAS motif within a modified A domain. Our crystal struct
ure will allow reliable models to be built for other members of the A
domain superfamily and should facilitate development of novel adhesion
modulatory drugs.