The systemic toxicity of tris(4-chlorophenyl)methanol (TCPM) was studi
ed in male and female rats following 4 weeks dietary exposure dosed at
1, 10 and 100 ppm. An increased spleen to body weight ratio was obser
ved in males at 10 and 100 ppm and in females at 100 ppm. An increased
liver to body weight ratio was detected in both sexes at 100 ppm. Dos
e-related increases in hepatic Phase-I (AH, APDM, EROD and PROD) and P
hase-II (UDPGT, GST) enzyme activities were observed generally at 10 a
nd 100 ppm, with the elevation in PROD activity being the most marked.
Increased urinary ascorbic acid was detected in both males and female
s after 1 week of treatment at 100 ppm and after 4 weeks of treatment
at 10 and 100 ppm. At 10 and 100 ppm, elevated % lymphocytes were foun
d in males, and higher white blood cell and lymphocyte counts were obs
erved in females. In the liver, mild to moderate cytoplasmic changes c
onsistent with proliferation of smooth endoplasmic reticulum were pres
ent in rats of both sexes at 10 and 100 ppm, and increased number of h
epatocytes undergoing apoptosis were observed in male rats at 100 ppm.
Mild splenic changes consisting of sinus hyperplasia in males and fem
ales at 100 ppm and mantle zone atrophy in males at 100 ppm were also
observed. It was concluded that TCPM at a dietary concentration of 10
ppm (equivalent to 1.2 mg/kg/day) produced systemic changes in rats th
at included various hepatic effects, increased splenic weight, and mod
ulations in white blood cells and lymphocyte counts. Copyright (C) 199
6 Elsevier Science Ltd