J. Salinas et al., ANTIGENIC DIVERSITY OF LUMINANT CHLAMYDIA-PSITTACI STRAINS DEMONSTRATED BY THE INDIRECT MICROIMMUNOFLUORESCENCE TEST WITH MONOCLONAL-ANTIBODIES, Veterinary microbiology, 43(2-3), 1995, pp. 219-226
Monoclonal antibodies (mAbs) were produced to find strain markers esse
ntial to the epidemiological study of chlamydial abortion of ruminants
. Their specificity was tested on 53 C. psittaci strains including 35
ruminant strains isolated mainly from abortion, belonging to serotype
1 and which are invasive in our mouse model (Rodolakis et al., 1989),
and 14 ruminant strains mostly intestinal, belonging to serotype 2 and
non-invasive for mouse. One strain specific mAb was obtained reacting
only with the non-invasive strain iB1. Six sub serotype 2 mAbs were f
ound. They reacted only with some non-invasive strains. They allowed t
he distinction of 9 patterns of response among the 14 noninvasive stra
ins. No serotype 2 specific mAbs reacting with all non-invasive seroty
pe 2 strains were selected. In return all the invasive strains reacted
with all the 18 serotype 1 specific mAbs produced. No cross-reactivit
ies between invasive and non-invasive strains were observed, whereas c
ommon epitopes were demonstrated between invasive strains and avian or
feline strains.