ANTIGENIC DIVERSITY OF LUMINANT CHLAMYDIA-PSITTACI STRAINS DEMONSTRATED BY THE INDIRECT MICROIMMUNOFLUORESCENCE TEST WITH MONOCLONAL-ANTIBODIES

Citation
J. Salinas et al., ANTIGENIC DIVERSITY OF LUMINANT CHLAMYDIA-PSITTACI STRAINS DEMONSTRATED BY THE INDIRECT MICROIMMUNOFLUORESCENCE TEST WITH MONOCLONAL-ANTIBODIES, Veterinary microbiology, 43(2-3), 1995, pp. 219-226
Citations number
30
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
ISSN journal
03781135
Volume
43
Issue
2-3
Year of publication
1995
Pages
219 - 226
Database
ISI
SICI code
0378-1135(1995)43:2-3<219:ADOLCS>2.0.ZU;2-P
Abstract
Monoclonal antibodies (mAbs) were produced to find strain markers esse ntial to the epidemiological study of chlamydial abortion of ruminants . Their specificity was tested on 53 C. psittaci strains including 35 ruminant strains isolated mainly from abortion, belonging to serotype 1 and which are invasive in our mouse model (Rodolakis et al., 1989), and 14 ruminant strains mostly intestinal, belonging to serotype 2 and non-invasive for mouse. One strain specific mAb was obtained reacting only with the non-invasive strain iB1. Six sub serotype 2 mAbs were f ound. They reacted only with some non-invasive strains. They allowed t he distinction of 9 patterns of response among the 14 noninvasive stra ins. No serotype 2 specific mAbs reacting with all non-invasive seroty pe 2 strains were selected. In return all the invasive strains reacted with all the 18 serotype 1 specific mAbs produced. No cross-reactivit ies between invasive and non-invasive strains were observed, whereas c ommon epitopes were demonstrated between invasive strains and avian or feline strains.