Wj. Underwood et al., PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO BOVINE RESPIRATORY SYNCYTIAL VIRUS, Veterinary microbiology, 43(2-3), 1995, pp. 261-274
Monoclonal antibodies to the SmithKIine Beecham Animal Health BRSV(R)
vaccine strain (375 isolate of Bovine Respiratory Syncytial Virus) wer
e produced and then characterized by radioimmunoprecipitation followed
by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, virus n
eutralization, inhibition of viral-induced fusion and isotype. Ninetee
n hybridomas produced antibodies that were reactive with the F-0, F-1;
N or P viral. proteins of bovine respiratory syncytial virus. One hyb
ridoma (1E72C4) produced antibodies that immunoprecipitated the F-0 gl
ycoprotein, neutralized virus (1:8) in the presence of complement, but
did not inhibit fusion. Another hybridoma (8B21E7) produced antibodie
s that immunoprecipitated the F-0, F-1 and F-2 glycoproteins, neutrali
zed virus (1:4) with and without complement and inhibited fusion, Anti
bodies from 11 hybridomas immunoprecipitated N and F-1 proteins, one h
ybridoma immunoprecipitated the N and P protein, and 5 hybridomas immu
noprecipitated the N protein. All monoclonal antibodies were of the Ig
G(2b) subtype with either kappa or lambda light chains.