PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO BOVINE RESPIRATORY SYNCYTIAL VIRUS

Monoclonal antibodies to the SmithKIine Beecham Animal Health BRSV(R) vaccine strain (375 isolate of Bovine Respiratory Syncytial Virus) wer e produced and then characterized by radioimmunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, virus n eutralization, inhibition of viral-induced fusion and isotype. Ninetee n hybridomas produced antibodies that were reactive with the F-0, F-1; N or P viral. proteins of bovine respiratory syncytial virus. One hyb ridoma (1E72C4) produced antibodies that immunoprecipitated the F-0 gl ycoprotein, neutralized virus (1:8) in the presence of complement, but did not inhibit fusion. Another hybridoma (8B21E7) produced antibodie s that immunoprecipitated the F-0, F-1 and F-2 glycoproteins, neutrali zed virus (1:4) with and without complement and inhibited fusion, Anti bodies from 11 hybridomas immunoprecipitated N and F-1 proteins, one h ybridoma immunoprecipitated the N and P protein, and 5 hybridomas immu noprecipitated the N protein. All monoclonal antibodies were of the Ig G(2b) subtype with either kappa or lambda light chains.