CHARACTERIZATION OF ANTHRAQUINONE-2-CARBONYL CHLORIDE AS AN ALCOHOL DERIVATIZATION REAGENT FOR NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY

Anthraquinone-2-carbonyl chloride has been utilized as a derivatizatio n reagent for alcohols to impart electron affinity and aid in transpor t via a particle beam liquid chromatography-mass spectrometry (LC/MS) interface. In addition, the gas chromatographic-mass spectrometry, UV, fluorescence, and electrochemical characteristics of the derivatives were determined. A series of model compounds, 2-phenylethanol (pheneth yl alcohol), 1-phenyl-2-propanol, 2-methyl-1-phenyl-2-propanol, hexano l, and methyl 2-methylglycerate, were used as analytes. The particle b eam LC/MS properties of the resultant anthraquinone carboxylate esters were determined in electron impact (EI) and negative ion chemical ion ization (NCI) modes. The NCI responses of these anthraquinone carboxyl ate esters were compared with the corresponding 3,5-dinitrobenzoate es ters. The anthraquinone carboxylate esters exhibited an NCI to EI sens itivity enhancement of 113 and were detected in NCI at a tenfold lower concentration than the corresponding 3,5-dinitrobenzoate esters. A de tection limit of 26 pg injected on column was achieved for phenethyl a nthraquinone carboxylate in NCI by using selected ion monitoring.