CLONING OF NISHIKI-GOI, JAPANESE ORNAMENTAL (KOI) CARP

Endomitosis was induced by inseminating eggs of a koi female with UV-i rradiated (800J/m2) milt of a wild-type colored common carp male and i ncubating them at 23-degrees-C. Diploidy was restored by exposing the eggs to a ''late'' heat shock (30-48 min after insemination at 40.0 +/ - 0.5-degrees-C for 2 min). The wild-type coloration of the milt donor served as a dominant marker. Only 14 of these mitogynotes survived to sexual maturity. Three of the females were used to produce genetic cl ones by gynogenesis. Meiotic gynogenesis was induced by inseminating t heir eggs with UV-irradiated milt. Diploidy was restored by ''early'' pressure shock applied 5 min after insemination (7500-7600 psi for 2 m in). Survival in three different batches of these meiogynotes was betw een 60% and 21%, much higher than that of the mitogynotes in the previ ous generation.