MEMBRANE FLUIDITY MEMBRANE LIPID PATTERN AND CYTOSOLIC CA2-II DIABETIC-PATIENTS WITH MACROVASCULAR COMPLICATIONS( CONTENT IN PLATELETS FROMA GROUP OF TYPE)
G. Caimi et al., MEMBRANE FLUIDITY MEMBRANE LIPID PATTERN AND CYTOSOLIC CA2-II DIABETIC-PATIENTS WITH MACROVASCULAR COMPLICATIONS( CONTENT IN PLATELETS FROMA GROUP OF TYPE), Diabetes care, 18(1), 1995, pp. 60-63
Citations number
31
Categorie Soggetti
Endocrynology & Metabolism","Medicine, General & Internal
OBJECTIVE - To evaluate platelet membrane fluidity and some platelet m
etabolic parameters in type II diabetic patients with macrovascular co
mplications. RESEARCH DESIGN AND METHODS - In a group of 21 type II di
abetic patients with macrovascular complications, we evaluated platele
t membrane fluidity [marking intact resting platelets with the fluores
cent probe 1,4-(trimethylamino) phenyl-4-phenylhexatriene (TMA-DPH)],
platelet membrane lipid pattern (cholesterol:phospholipid [C:PL] ratio
and individual phospholipids), and platelet cytosolic Ca2+ content (m
arking intact resting platelets with the fluorescent probe Fura 2AM).
RESULTS - Platelet membrane fluidity is decreased in type II diabetic
patients with macrovascular complications compared with normal subject
s (P < 0.001). Platelet membrane C:PL ratio and cytosolic Ca2+ content
do not discriminate normal subjects from diabetic patients, and for i
ndividual phospholipids, only phosphatidylethanolamine is decreased in
diabetic patients compared with control subjects (P = 0.051). In norm
al subjects, the polarization degree of TMA-DPH is related to phosphat
idylserine (P < 0.05) and phosphatidylcholine (P < 0.05), and in diabe
tic patients the polarization degree of TMA-DPH is related to C:PL rat
io (P < 0.05) and sphyngomyelin (P < 0.05). CONCLUSIONS - In type II d
iabetic patients with macrovascular complications, we observed an abno
rmality of platelet membrane fluidity, which may contribute to platele
t functional alteration present in this clinical condition.