Mrb. Puncher et Pj. Blower, LABELING OF LEUKOCYTES WITH COLLOIDAL TECHNETIUM-99M-SNF2 - AN INVESTIGATION OF THE LABELING PROCESS BY AUTORADIOGRAPHY, European journal of nuclear medicine, 22(2), 1995, pp. 101-107
Autoradiography of smears and frozen sections of labelled cell suspens
ions was used to study the distribution of radioactivity in and among
blood cells labelled in either whole blood or leucocyte-rich plasma (L
RP) with technetium-99m-SnF2 colloid. The tracer proved selective for
neutrophils: the labelling probability (relative to that for erythrocy
tes) for each cell type in LRP (mean of five samples) was: neutrophils
, 9.4; lymphocytes, 3.7; monocytes, 3.0; eosinophils 1.4 erythrocytes,
1.0. When labelling was carried out rn whole blood (five samples), 74
.5%+/-8.3% of the cell-bound radioactivity was bound to erythrocytes,
13.6%+/-6.5% to neutrophils, and 11.9%+/-2.1% to lymphocytes, whereas
in LRP (in which the leucocytes were only slightly outnumbered by eryt
hrocytes), 76.5%+/-14.9% of radioactivity was neutrophil bound. Labell
ed cells in smear autoradiographs exhibited two distinct silver grain
pat terns, ''diffuse'', consistent with an intracellular radioactive p
article (in neutrophils), and ''focal'', consistent with a cell surfac
e-adhering particle in direct contact with the emulsion (in other leuc
ocyte types and erythrocytes). The phagocytic inhibitor cytochalasin B
neither reduced the proportion of labelled neutrophils nor altered th
e labelling pattern. Neutrophils were able to scavenge radioactivity f
rom the surface of erythrocytes. It is concluded that neutrophils bind
Tc-99m-SnF2 intracellularly by phagocytosis, with high affinity; othe
r cells become labelled at the cell surface reversibly and with lower
affinity. This selectivity is high enough to permit predominantly leuc
ocyte labelling in LRP but not in whole blood.